The angry women lured married father Donessa Davis to a motel where they decided to humiliate him.
The ambush was set up by the man's wife after she discovered he had been cheating on her with as many as five women.
Police in Wisconsin are now searching for the scorned wife after she disappeared from the family home after the assault.
Mr Davis is recovering after needing hospital treatment for his embarrassing injury when his manhood was glued to his stomach.
The attack took place after Therese Ziemann, 47, lured the husband to a motel in Chilton, Wisconsin, with the promise of a massage.
The husband voluntarily allowed himself to be tied to the bed and blindfolded.
As he lay prone on the bed, Ziemann opened the motel room door and two other women, Wendy Sewell, 44, and Michelle Belliveau, 43, burst in.
All three had been contacted by the Mr Davis' wife Tracy Hood-Davis. She had discovered her husband's affairs and had plotted with them to exact their revenge.